In Night Shift Nurses, Ryuji Hirasaka, a middle-aged Japanese gynecologist, finds himself unemployed, single, and living independently. One evening, he receives an email from St. Juliana Hospital, a fictional local institution, offering him a temporary job opportunity. He is shocked to discover that his potential employer is none other than Narumi Jinguji, a woman he had brutally assaulted in the past. Despite the tense atmosphere, Narumi reveals her desire to establish a department in the hospital dedicated to addressing the "special" needs of patients and seeks Ryuji's expertise to oversee its operation. Overwhelmed with lewd excitement, Ryuji agrees to her proposal.